Scientist · Innovator · Builder

Imaging the
invisible.
Building what's
missing.

Advanced microscopy, optics, and scientific instrumentation across the boundary between what can be seen and what can be built.

Oral Talk 29th PhotoIUPAC Symposium on Photochemistry IUPAC · July 2024 · Spain
25 Publications
610+ Citations
19+ Talks
5 Microscopes Built
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"The best scientific instruments are the ones that make previously impossible questions suddenly answerable."

— Research Philosophy

I am a researcher and instrument builder working at the frontier of optical microscopy, nanoscience, and advanced imaging. My work spans fundamental physics and practical engineering — designing new systems that reveal how matter behaves at scales the naked eye cannot reach.

Beyond the lab, I build tools and platforms for the broader scientific community, including MicroscopyIndex.io, an open resource for microscopy researchers worldwide. I am driven by a conviction that science advances fastest when knowledge and tools are shared freely and built well.

Microscopy Optics & Photonics Nanoscience Instrumentation Imaging Scientific Tools
Research Focus

What I work on

My work spans several interconnected domains, united by a shared interest in seeing and measuring matter at its most fundamental scales.

01

Optical Microscopy & Instrumentation

Designing, building, and operating custom microscopy systems: from multiplane and multidimensional platforms to optical trapping setups. Three instruments built and fully operational, two more in progress.

02

Image Processing & Analysis

Developing algorithms that extract quantitative information from complex imaging data, including CLIM for functional mapping of materials, 3D particle tracking, and open-source tools for the community. Actively learning and developing deep learning tools

03

Optical Manipulation

Using structured light fields to trap, assemble, and control matter at the nanoscale, from optical tweezers and optical binding to the emergent physics of light-driven nanoparticle assemblies. Also working on dielectrophoretic manipulation which uses alternating electric field instead of light.

04

Nanoscience & Materials

Investigating functional materials such as perovskites, colloidal nanoparticles, optoelectronic devices, at the nanoscale, where optical properties, carrier dynamics, and structural heterogeneity determine macro behaviour.

Selected Work

Featured projects

01
Instrumentation · System Design
Multidimensional Microscopy Platform
Custom optical system enabling high-resolution simultaneous spatial and spectral imaging.
02
Nanoscience · Optics
Optical Matter Dynamics
Light-driven assembly and dynamics of nanoparticle systems in structured optical fields.
03
Methods · Computation
Correlation Clustering Imaging
Method that exploits spatiotemporal intensity fluctuations to investigate solar cell materials and their properties.
04
Open Platform
MicroscopyIndex.io
An open resource and community repository for the microscopy research community worldwide.
Research Output

Selected publications

High Resolution Imaging of Nonequilibrium Colloidal Self-Assembly via Photofixation
J. Satpathy, Jim J-K. Chen, G. Wen, H. Masuhara, S. Seth, V. Leen, S. Rocha, J. Hofkens, B. Louis, and Roger Bresolí-Obach· ACS Nano · 2025  †co-corresponding author
View Full Publication List

Major Initiative

Open tools for the imaging community

microscopyindex.io

MicroscopyIndex.io is an open repository and community resource for microscopy researchers worldwide, a central hub for instruments, protocols, datasets, and knowledge that has long been scattered across the literature.

Learn More

Curated Repository

Structured, searchable access to instruments, protocols, and resources.

Community-Driven

Built with and for the global microscopy research community.

Open Access

Free, open, and designed to lower barriers across the field.

Work Together

How I can help

I am open to a range of collaborations, from scientific partnerships to consulting, speaking, and joint initiatives.

Research Collaboration

Interdisciplinary projects combining optical science, imaging, and materials research.

Industry Consulting

Optics, microscopy systems, imaging pipelines, and scientific instrumentation.

Invited Talks

Conferences, symposia, workshops, and seminars on microscopy, imaging, and open science.

Open Science

Partnerships around open tools, community platforms, and shared scientific infrastructure.

Start a Conversation
Background

I study how light reveals the structure of matter, and build the tools to do it better.

My research sits at the intersection of optical physics, instrument design, and nanoscience. I am interested in questions that require new tools to answer, and in building those tools with the care and rigor that science demands.

I have worked on problems ranging from the design of advanced microscopy platforms to the physics of optically driven nanoparticle assemblies. What connects these threads is a conviction that the quality of scientific instrumentation directly determines the quality of the science it enables.

Beyond the lab, I think seriously about the infrastructure of science itself, how tools, data, and knowledge are shared, and I build accordingly.

Research Interests

My work spans advanced microscopy system design, multidimensional imaging, optical trapping, optical matter, nanophotonics, and precision instrumentation. I am drawn to problems that sit at the edge of what existing tools can measure, and to the engineering challenges of extending those limits.

I approach instrumentation not just as an engineering exercise, but as a form of scientific reasoning: the design of a tool encodes assumptions about what matters and what can be known.

Education & Career
Present

Researcher — KU Leuven, Molecular imaging and photonics, Hofkens laboratory

Advanced microscopy, optical instrumentation, multidimensional imaging, and the MicroscopyIndex.io platform.

2022

Ph.D. of Science In Chemistry, KU Leuven/Lund University

Thesis: Multidimensional microscopy a versatile tool to unravel material properties at the nanoscale]

2016

M.Sc. Chemistry, Université de Liège

Summa cum Laude. One year Erasmus in Lund University, One year Erasmus Belgica at KU Leuven

2014

B.Sc. Chemistry, Université de Liège

Magna Cum Laude

Beyond the Lab

I am a strong believer in open science, collaborative knowledge-building, and the long-term importance of scientific infrastructure. MicroscopyIndex.io reflects this belief in practice. I am also interested in the relationship between scientific tools and scientific culture — how the instruments a field uses shape the questions it asks.

I am open to conversations about research collaborations, consulting, invited talks, or joint initiatives that advance the quality and accessibility of scientific tools.

Research Focus

Focus areas

01

Optical Microscopy & Instrumentation

Designing, building, and operating custom microscopy systems — from multiplane and multidimensional platforms to optical trapping setups. Three instruments fully operational, two more in progress.

02

Image Processing & Analysis

Developing algorithms that extract quantitative information from complex imaging data — including CLIM for functional mapping of materials, 3D particle tracking, and open-source tools for the community.

03

Optical Manipulation

Using structured light fields to trap, assemble, and control matter at the nanoscale — from optical tweezers and optical binding to the emergent physics of light-driven nanoparticle assemblies.

04

Nanoscience & Materials

Investigating functional materials — perovskites, colloidal nanoparticles, optoelectronic devices — at the nanoscale, where optical properties, carrier dynamics, and heterogeneity determine device behaviour.

Research Lines

Active research lines

Three research programmes I lead or co-lead at the Hofkens Laboratory, KU Leuven — each addressing a distinct scientific question at the intersection of optics, imaging, and nanoscience.

01
Optical Physics · Nanoscience
Optical Matter & Nanoparticle Assembly
How do light fields drive the collective organisation of nanoparticles into functional structures?
Using optical forces to trap, bind, and assemble colloidal nanoparticles into organised structures — exploring the emergent physics of light-matter interaction beyond the irradiation zone. I co-supervise three PhD students working on distinct aspects of this problem.
Optical Tweezers Optical Binding Chiral Assemblies Particle Shape Collective Dynamics
02
Imaging · Materials Science
Functional Imaging of Optoelectronic Devices
How do nanoscale heterogeneities in solar cell materials determine macroscopic device efficiency?
Developing and applying advanced imaging methods — including CLIM, super-resolution microscopy, and deep learning — to map functional domains in halide perovskite films and other optoelectronic materials. This line bridges fundamental photophysics with applied materials design.
CLIM Perovskites Super-resolution Deep Learning Solar Cells
03
Nanoscience · Data Storage
Colloidal Memory & Data Storage
Can colloidal nanoparticle systems encode, store, and retrieve information at the nanoscale?
Exploring colloidal nanoparticles as a medium for data storage — investigating encoding strategies, optical readout mechanisms, and the physical limits of information density at the nanoscale. A long-term fundamental research programme with significant technological implications.
Colloidal Nanoparticles Data Encoding Optical Readout Nanoscale Storage
Projects

Active & recent work

01
Instrumentation · System Design
Multidimensional Microscopy Platform
Custom optical system enabling high-resolution simultaneous spatial and spectral imaging.
02
Nanoscience · Optics
Optical Matter Dynamics
Light-driven assembly and dynamics of nanoparticle systems in structured optical fields.
03
Methods · Computation
Correlation Clustering Imaging
Method that exploit spatiotemporal intensity fluctuations to investigate solarcell materials and their properties.
04
Open Platform
MicroscopyIndex.io
An open resource and community repository for the microscopy research community worldwide. [Under Development]

Selected Highlights

Full List

2026
High Resolution Imaging of Nonequilibrium Colloidal Self-Assembly via Photofixation
J. Satpathy, J. J.-K. Chen, G. Wen, H. Masuhara, S. Seth, V. Leen, S. Rocha, J. Hofkens, B. Louis*, R. Bresolí-Obach* · ACS Nano · 2026
Tunable Optical Matter: Electrostatic Repulsion Modulates Near- and Far-Field Gold Nanoparticle Arrangements
J. J.-K. Chen, J. Olmos-Trigo, B. Louis, C.-H. Huang, S. Rocha, H. Masuhara, J. Hofkens, R. Delgado-Buscalioni, R. Bresolí-Obach, M. Marqués, M. Melendez Schofield · Nanoscale Advances · 2026
2025
Intracellular Dynamics and Photodynamic Efficacy of Black Phosphorus Quantum Dots in Cancer Models
S. Zaman, B. Fortuni, B. Louis, S. Brooke, S. Berden, P. Mulvaney, J. Hutchison, S. Rocha · Materials & Design, 260, 115044 · 2025
Programmable Shape-Directed Optical Binding of Plasmonic Nanoparticles
J. J.-K. Chen, A. Kar, P. Yu, A. Sánchez-Iglesias, C.-H. Huang, J. Satpathy, J. Wang, L. M. Liz-Marzán, H. Masuhara, R. Bresolí-Obach, S. Seth, S. Rocha, B. Louis*, J. Hofkens · Advanced Optical Materials · 2025
Applying 8-Foci Imaging to Instantaneous Three-Dimensional Flow Field Reconstruction
A. Gholivand, R. Bresolí-Obach, B. Louis, K. Dahlhoff, T. Dickscheid, J. Hofkens, M. P. Lettinga · Physics of Fluids, 37, 112010 · 2025
Interfacial Assembly of Dye-Doped Microparticles Driven by Combined Optical and Non-Optical Forces
Q. Q. Wang, J. J.-K. Chen, Y.-C. Chang, B. Louis, R. Delgado-Buscalioni, S. Toyouchi, H. Masuhara, S. Rocha, J. Hofkens, R. Bresolí-Obach · Advanced Optical Materials · 2025
Morphology Control of Dynamic Optical Matter of Gold Nanoparticles Fabricated by Optical Trapping in Printed Microchannels
P.-H. Huang, M.-E. Li, C.-S. Lu, C.-H. Huang, H.-N. Wu, P.-C. Tsai, J. J.-K. Chen, B. Louis, R. Bresolí-Obach, S. Rocha, J. Hofkens, H. Witek, M.-C. Li, H. Masuhara · Photochemical & Photobiological Sciences, 24, 751–764 · 2025
In Operando Locally-Resolved Photophysics in Perovskite Solar Cells by Correlation Clustering Imaging
B. Louis, S. Seth, Q. An, R. Ji, Y. Vaynzof, J. Hofkens, I. G. Scheblykin · Advanced Materials, 37, 2413126 · 2025
Unveiling the Local Fate of Charge Carriers in Halide Perovskite Thin Films via Correlation Clustering Imaging
S. Seth, B. Louis, K. Asano, T. Van Roy, M. B. J. Roeffaers, E. Debroye, I. G. Scheblykin, M. Vacha, J. Hofkens · Chemical & Biomedical Imaging · 2025
2024
Unconventional Optical Matter of Hybrid Metal–Dielectric Nanoparticles at Interfaces
B. Louis, C.-H. Huang, M. Melendez, A. Sánchez-Iglesias, J. Olmos-Trigo, S. Seth, S. Rocha, R. Delgado-Buscalioni, L. M. Liz-Marzán, M. I. Marqués, H. Masuhara, J. Hofkens, R. Bresolí-Obach · ACS Nano, 18, 32746–32758 · 2024
Plasmonic Dipole and Quadrupole Scattering Modes Determine Optical Trapping, Optical Binding, and Swarming of Gold Nanoparticles
C.-H. Huang, B. Louis, S. Rocha, L. M. Liz-Marzán, H. Masuhara, J. Hofkens, R. Bresolí-Obach · The Journal of Physical Chemistry C, 128, 5731–5740 · 2024
2023
Synthetic Fibrous Hydrogels as a Platform to Decipher Cell–Matrix Mechanical Interactions
H. Yuan, K. Liu, M. Cóndor, J. Barrasa-Fano, B. Louis, J. Vandaele, P. de Almeida, Q. Coucke, W. Chen, E. Oosterwijk, C. Xing, H. Van Oosterwyck, P. H. J. Kouwer, S. Rocha · Proceedings of the National Academy of Sciences, 120, e2216934120 · 2023
Unravelling 3D Dynamics and Hydrodynamics during Incorporation of Dielectric Particles to an Optical Trapping Site
B. Louis, C.-H. Huang, R. Camacho, I. G. Scheblykin, T. Sugiyama, T. Kudo, M. Melendez, R. Delgado-Buscalioni, H. Masuhara, J. Hofkens, R. Bresolí-Obach · ACS Nano, 17, 3797–3808 · 2023
Gaining Control on Optical Force by the Stimulated-Emission Resonance Effect
T. Kudo, B. Louis, H. Sotome, J.-K. Chen, S. Ito, H. Miyasaka, H. Masuhara, J. Hofkens, R. Bresolí-Obach · Chemical Science, 14, 10087–10095 · 2023
2022
The Primeval Optical Evolving Matter by Optical Binding Inside and Outside the Photon Beam
C.-H. Huang, B. Louis, R. Bresolí-Obach, T. Kudo, R. Camacho, I. G. Scheblykin, T. Sugiyama, J. Hofkens, H. Masuhara · Nature Communications, 13, 5325 · 2022 equal contribution
Expansion Microscopy Allows High Resolution Single Cell Analysis of Epigenetic Readers
A. Acke, S. Van Belle, B. Louis, R. Vitale, S. Rocha, T. Voet, Z. Debyser, J. Hofkens · Nucleic Acids Research, 50, e100 · 2022
Ladder-like Polymer Brushes Containing Conjugated Poly(Propylenedioxythiophene) Chains
G. Grześ, K. Wolski, T. Uchacz, J. Bała, B. Louis, I. G. Scheblykin, S. Zapotoczny · International Journal of Molecular Sciences, 23, 5886 · 2022
2021
Heterogeneities and Emissive Defects in MAPbI₃ Perovskite Revealed by Spectrally Resolved Luminescence Blinking
M. Gerhard, B. Louis, P. A. Frantsuzov, J. Li, A. Kiligaridis, J. Hofkens, I. G. Scheblykin · Advanced Optical Materials, 9, 2001380 · 2021
Resonantly Enhanced Optical Trapping of Single Dye-Doped Particles at an Interface
R. Bresolí-Obach, T. Kudo, B. Louis, Y. C. Chang, I. G. Scheblykin, H. Masuhara, J. Hofkens · ACS Photonics, 8, 1832–1839 · 2021
2020
Fast-Tracking of Single Emitters in Large Volumes with Nanometer Precision
B. Louis, R. Camacho, R. Bresolí-Obach, S. Abakumov, J. Vandaele, T. Kudo, H. Masuhara, I. G. Scheblykin, J. Hofkens, S. Rocha · Optics Express, 28, 28656 · 2020
Optical Force-Induced Dynamics of Assembling, Rearrangement, and Three-Dimensional Pistol-like Ejection of Microparticles at the Solution Surface
J. S. Lu, T. Kudo, B. Louis, R. Bresolí-Obach, I. G. Scheblykin, J. Hofkens, H. Masuhara · The Journal of Physical Chemistry C · 2020
Structural Characterization of Fibrous Synthetic Hydrogels Using Fluorescence Microscopy
J. Vandaele, B. Louis, K. Liu, R. Camacho, P. H. J. Kouwer, S. Rocha · Soft Matter, 16, 4210–4219 · 2020
2019
Microscopic Insight into Non-Radiative Decay in Perovskite Semiconductors from Temperature-Dependent Luminescence Blinking
M. Gerhard, B. Louis, R. Camacho, A. Merdasa, J. Li, A. Kiligaridis, A. Dobrovolsky, J. Hofkens, I. G. Scheblykin · Nature Communications, 10, 1698 · 2019 equal contribution
Impact of Excess Lead Iodide on the Recombination Kinetics in Metal Halide Perovskites
A. Merdasa, A. Kiligaridis, C. Rehermann, M. Abdi-Jalebi, J. Stöber, B. Louis, M. Gerhard, S. D. Stranks, E. L. Unger, I. G. Scheblykin · ACS Energy Letters, 4, 1370–1378 · 2019
2018
Light and Oxygen Induce Chain Scission of Conjugated Polymers in Solution
B. Louis, S. Caubergh, P. O. Larsson, Y. Tian, I. G. Scheblykin · Physical Chemistry Chemical Physics, 20, 1829–1837 · 2018

Preprints

M3Scope: A 3D Multimode Multiplane Microscope for Imaging Nanoscale Dynamics in Soft Matter
S. Huysecom, F. Bevilacqua, R. Bresolí-Obach, S. Seth, L. M. Liz-Marzán, D. R. D. Hooge, J. Hofkens, S. Rocha, B. Louis · arXiv:2511.10174 · 2025
Quantitative Analysis of Cytoplasmic Viscosity in Colorectal Cancer Cells by Differential Dynamic Microscopy of Genetically Encoded Nanoparticles
S. Huysecom, K. Schouwstra, S. Aytekin, N. Neyrinck, Y. Heyvaert, B. Louis, S. Rocha, G. Solís-Fernández · bioRxiv · 2025
Microscopic Intricacies of Self-Healing in Halide Perovskite–Charge Transport Layer Heterostructures
T. Behera, B. Louis, L. Paesen, R. Vanden Brande, K. Asano, M. Vacha, M. Roeffaers, E. Debroye, J. Hofkens, S. Seth · arXiv:2510.11948 · 2025
Absorption Force That Rules Them All: Chemical Control over Optical Trapping
L. Torella-Adriaensen, B. Louis, J. Satpathy, A. Tarrats, J. Rodrigo Magaña, C. Fornaguera, S. Nonell, J. Hofkens, R. Bresolí-Obach · ChemRxiv · 2025
Beyond One-Size-Fits-All: Tumor Biology Influences Nanoparticle Behaviour in Cancer Models
M. Bravo, G. Solís-Fernández, S. Krzyzowska, S. Huysecom, A. Montero-Calle, I. Van Zundert, B. Louis, R. Barderas, B. Fortuni, S. Rocha · bioRxiv · 2025
Three-Dimensional Optical Reconstruction of Colloidal Electrokinetics via Multiplane Imaging
F. de Jong, P. Diez-Silva, J.-K. Chen, R. Pérez-Peláez, S. Seth et al. · arXiv:2503.23839 · 2025

For a complete and up-to-date list, please visit my Google Scholar profile.

View on Google Scholar
What it is

The index that microscopy has always needed.

Microscopy is a vast and fragmented field. Instruments, protocols, datasets, analysis tools, and hard-won expertise are scattered across thousands of papers, lab wikis, and individual memories. MicroscopyIndex.io exists to bring this knowledge together in one open, structured, and community-maintained resource.

The platform is designed to be genuinely useful — searchable, well-organized, and built with the working scientist in mind. It is free, open-access, and built to last.

Why it matters

Science moves faster when tools are shared.

Every researcher who has spent weeks hunting for an obscure technique or rebuilding something another lab already solved understands the problem. The fragmentation of microscopy knowledge is not just an inconvenience — it is a structural barrier that slows discovery, particularly for labs with fewer resources.

MicroscopyIndex.io is a direct response to this gap — one built by someone who has felt it firsthand.

Platform Pillars

What the platform offers

Instrument Repository

Curated records of microscopy instruments — commercial and custom-built — with structured metadata, specifications, and links to primary literature.

Protocol Library

Step-by-step protocols, sample preparation methods, and best practices contributed and reviewed by the community.

Analysis Tools

An indexed collection of open-source analysis tools, plugins, and software pipelines relevant to microscopy researchers.

Community-Maintained

Built with contributions from researchers worldwide — designed to grow, improve, and remain accurate over time.

An open resource for anyone who works with light and lenses.

Visit MicroscopyIndex.io Partner With Us
Philosophy

I believe the most interesting science happens at boundaries — between disciplines, between academia and industry, between the lab and the world outside it. I am always interested in conversations that cross those boundaries.

Whether you are looking for a research collaborator, a speaker who can make imaging science accessible and compelling, or a technical advisor for an instrument or imaging challenge — I am open to hearing from you.

"The best collaborations are the ones where neither party could have arrived at the same place alone."
Opportunities

How we might work together

Speaking

Invited Talks & Lectures

I speak on microscopy, optical instrumentation, multidimensional imaging, and the future of open scientific tools. I am available for conferences, symposia, departmental seminars, and workshops.

Research

Scientific Collaboration

I welcome collaborations with researchers in adjacent fields who need advanced imaging or instrumentation expertise — or who work on problems that intersect with optics, photonics, or nanoscience.

Industry

Technical Consulting

I consult for companies developing optical instruments, imaging systems, or scientific hardware — from early-stage startups to established instrument manufacturers.

Partnerships

Open Science & Platform

I am interested in partnerships that support the development of MicroscopyIndex.io and broader efforts to build open infrastructure for the scientific community.

Teaching

Workshops & Training

Hands-on workshops on microscopy techniques, optical design, instrumentation, and image analysis for researchers at all career stages.

Interdisciplinary

Cross-Sector Projects

Exploratory conversations welcome. If you are working on a problem where advanced imaging or instrumentation might make a difference, let's talk.

I'm always open to interesting conversations.

Whether you want to discuss a collaboration, explore consulting, invite me to speak, or simply connect — I'd be glad to hear from you. I try to respond to all thoughtful enquiries.

Send a message